Dear Dr. Beth Moscato
I am measuring with AVIII using the following method.
F-H HOESY measurements can be made with the pulse program hoesyfhqfqnrv.
The following is the setup procedure.
(1) Load the parameters with the command rpar HCCOSW.
(*) This is for a totally unrelated pulse program, but it is a fast place to start.
② Execute command edsp to display the wiring screen.
③ Change 13C to 19F under F1 under NUC1.
④ Press Default button and close the Wiring screen by clicking Save (and Close) button.
⑤ Change pulprog to hoesyfhqfqnrv in the AcquPars tab.
Set the observation center, observation range, and totalization counts as appropriate.
(7) Execute the getprosol command to start the measurement.
In this procedure, 19F will be the observation side.
To set 1H as the observation side, press the 'Switch F1/F2' button in the wiring
screen between steps ③ and ④ to switch F1 and F2.
Whether 19F or 1H observation is better depends on the situation.
If the 19F range is large, 19F decoupling is not possible, so 19F observation
1H dec is better. On the other hand, 1H observation is more sensitive because
the 180-degree pulse hits properly.
It may be better to start with 1H observation for the first time.
However, in either case, if the range of the 19F signal is too wide, you may
not be able to get a good measurement.
Takahiro Kusukawa
Kyoto Institute of Technology, Kyoto, Japan
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Hi everyone –
I’m trying to set up a 1H / 19F HOESY on our AVANCE III HD instrument.
In the past, I’ve been told it’s an extremely difficult experiment
to set up (though it’s never been clear to me why), and I was never able
to get enough signal to noise from it on our NEO system at a previous job to make
it a useful tool, even for overnight analysis.
Does anyone have experience setting them up on a Bruker instrument? What are
the common failure modes?
I’ve managed to get both the 1D and 2D sequences to run without errors
on my instrument, but right now I’m getting absolutely no signal to
noise except for residual T1 noise in the 2D version. It’s possible
I just don’t have enough patience (and need to throw several thousand
scans at the problem or increase the concentration of my sample), but I’m
wondering if there’s something else I’m overlooking.
Thank you in advance!
Beth Moscato
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Received on Mon Mar 03 2025 - 16:38:20 MST