Hi Todd,
Adding to Youlin's thought, It could be that the HSQC experiment was being
run with more than the optimal acquisition time. That will make the
decoupler stay on for a long time and can cause the heating of the sample.
Best,
Atul
On Sat, Nov 2, 2024 at 3:58 PM Todd Rappe via groups.io wrote:
> A user in our lab was working optimizing a method and noticed that his 1H
> pulse width was changing after running an experiment. To confirm his
> observation, I ran a series of fast HSQC experiments on the same sample and
> ran pulsecal before each. The results are below:
>
> exp# P1, us
> 2 14.27
> 3 14.85
> 4 14.73
> 5 14.69
> 6 14.87
> 7 15.00
> 8 15.21
> 9 15.57
> 10 15.20
> 11 15.17
> 12 15.06
>
> I checked the tuning between each experiment and the wobble curve did not
> move at all.
>
> I followed up with simply running pulsecal several times in a row to see
> if it is just normal variability in the algorithm, but it gave me a
> consistent value +/- 0.01us each time.
>
> These tests were performed with a 15N-labeled protein sample in a 5-mm
> Shigemi tube on a 600-MHz Bruker Avance NEO w/ TCI cryoprobe.
>
> Does anybody have suggestions what could cause this variability? Thanks
>
>
>
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Received on Sun Nov 03 2024 - 08:20:01 MST