Dear Jay,
It sounds like the routing connections for the experiment don't match what
you've got. I don't have AVII experience, but for newer models, here's what
I'd do:
First, check to make sure in 'edhead' that you've got the right
routings/cablings to-from the probe in 'Edit RF Connections'. Pay extra
attention to the preamplifier slice that goes to the probe.
Then, run 'expinstall'. Wait for all the experiments to be installed.
If your carbon experiment is not the standard Bruker one, run 'paracon' on
it and other user sets.
At this point, you should be able to open a carbon parameter set, run it in
Topspin, and get what you're expecting.
Hope this helps!
Peter
On Tue, Jun 18, 2024 at 2:24 PM Shore, Jay via groups.io wrote:
>
> Thank you to everyone who responded to my original email.
> I feel like this group has my back.
> I apologize for not including more information.
> Our system is an Avance II running Topspin 2.1
>
> I did use edhead and it is the correct probe
> When saving using setprosol I told it to save to all solvents
> The “ignore proscol parameters” switch in ICONNMR was NOT activated
>
> I was making the mistake of setting up a run in iconnmr and before running
> it checking the parameters. I also made sure to save the parameters to the
> probe when using setprosol and saving for all solvents.
>
> Where it stands now.
>
> I can get a great 1H spectrum using iconnmr or topspin.
>
> I can NOT get a good 13C spectrum using iconnmr. If I go to the iconnmr
> created spectrum, type “ii” and then run in topspin, there is still no
> signal.
>
> I do have a file that has a beautiful 13C spectrum. If I go to it and type
> “ii” and run in topspin, I get another beautiful spectrum.
>
> I compared the parameters in eda line by line for the good and bad spectra
> and I do not see any difference.
>
> If I go to the spectrum created by iconnmr and type “edsp” I get “Some
> routing parameters in the file ‘acqu’ are not consistent with parameters
> saved in the file ‘specpar’. Acquistion will not work if you do not save
> the correct routing.” I also get “inconsistent observe routing for channel
> 1” I do not get these warnings if I go to the files with the nice spectra.
>
> Does anyone have any ideas.
>
> Thank you all in advance.
>
> Jay
>
> *Jay Shore*
> *Associate Professor*
> *Chemistry & Biochemistry & Physics*
>
> Avera Health & Science 355, Box 2202
> Brookings, SD 57007
>
> *P:* (605) 688-6356
> SDState.edu <https://www.sdstate.edu/>
>
>
--
*Peter Thompson, Ph.D.*
Lead Research Scientist, Molecular Education, Technology and Research Innovation Center (METRIC)
Research Assistant Professor, Department of Molecular and Structural Biochemistry
North Carolina State University
51 Polk Hall
Raleigh, NC 27695
Phone: 919.515.0836
Email: pmthomps_at_ncsu.edu
METRIC Website: https://research.ncsu.edu/metric/home
Consultation and Sample Submission: https://labs.ncsu.edu
Biochemistry Website:
https://cals.ncsu.edu/molecular-and-structural-biochemistry/
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Received on Tue Jun 18 2024 - 11:50:40 MST