Thank you to everyone who responded to my original email.
I feel like this group has my back.
I apologize for not including more information.
Our system is an Avance II running Topspin 2.1
I did use edhead and it is the correct probe
When saving using setprosol I told it to save to all solvents
The “ignore proscol parameters” switch in ICONNMR was NOT activated
I was making the mistake of setting up a run in iconnmr and before running
it checking the parameters. I also made sure to save the parameters to the
probe when using setprosol and saving for all solvents.
Where it stands now.
I can get a great 1H spectrum using iconnmr or topspin.
I can NOT get a good 13C spectrum using iconnmr. If I go to the iconnmr created
spectrum, type “ii” and then run in topspin, there is still no signal.
I do have a file that has a beautiful 13C spectrum. If I go to it and type
“ii” and run in topspin, I get another beautiful spectrum.
I compared the parameters in eda line by line for the good and bad spectra
and I do not see any difference.
If I go to the spectrum created by iconnmr and type “edsp”
I get “Some routing parameters in the file ‘acqu’ are
not consistent with parameters saved in the file ‘specpar’.
Acquistion will not work if you do not save the correct routing.”
I also get “inconsistent observe routing for channel 1” I
do not get these warnings if I go to the files with the nice spectra.
Does anyone have any ideas.
Thank you all in advance.
Jay
Jay Shore
Associate Professor
Chemistry & Biochemistry & Physics
Avera Health & Science 355, Box 2202
Brookings, SD 57007
P: (605) 688-6356
SDState.edu<
https://www.sdstate.edu/>
> From: main_at_ammrl.groups.io on behalf of Shore, Jay via groups.io
> Date: Sunday, June 16, 2024 at 7:32 PM
> To: main_at_ammrl.groups.io <main_at_ammrl.groups.io>
> `Subject: [AMMRL] edprosol conner
Okay … I am at my wits’ ends … again … which
was not a very for trip for me … seems like I am always in the neighborhood.
I changed probes on our Avance 600 from a PATXI to PABBO so that we could do
a wider range of nuclei (e.g., Pt).
Unfortunately, the bbo probe has never been calibrated (power and pulse lengths).
So, I went about calibrating and thought that as long as I saved the
new power levels and pulse lengths in the prosol file that they would show
up when I went to iconnmr. That is what the manual says. I saved the parameters
in the prosol file and saved it for the probe (all solvents). Then, I started
iconnmr (routine spectroscopy), set up an experiment, looked at the parameters
(for pulse length and power) and they were not the same as in the prosol file.
Does anyone have any ideas of what I am missing? Does anyone have a good reference
for calibrating deuterium power and pulse lengths or changing probes.
I would appreciate any advice.
Thank you.
Jay
Jay Shore
Associate Professor
Chemistry & Biochemistry & Physics
Avera Health & Science 355, Box 2202
Brookings, SD 57007
P: (605) 688-6356
SDState.edu<
https://www.sdstate.edu/>
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Received on Tue Jun 18 2024 - 11:24:46 MST