Re: [AMMRL] Help with 19F-1H HOESY Sequence on Varian Using Single Channel (Dual-Tuned Probe)

From: Spin-Doc <bert.heise_at_spin-doc.net>
Date: Tue, 22 Jul 2025 07:03:21 +0200
Dear Shawn,

Normally in VNMRS the correct channel routing from the two Ch1/2 transmitters to the highband amp happens inside the attenuator board (DD1) or inside the iCAT-transmitters over the short bridging cable (connecting Aux2 out -> Aux1 in). Remember to have all required 1H/19F pwx/pwxlvl calibrated in your probe file.
I’m not sure the Biopack HOESY sequence will work but the one in standard psglib (under “F-H experiments”) does - all spectra in /vnmr/fidlib/fluoropyrimidine were recorded on a standard 400MR (by me some 15 years back in our Oxford applab… ;-)).

Cheers
Bert

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Am 22.07.2025 um 01:06 schrieb Shawn Wagner via groups.io <shawn.wagner=usc.edu_at_groups.io>:

Hi all,
I'm trying to set up a 19F-1H HOESY experiment on a Varian NMR system using a dual-tuned high-band probe, where both 19F and 1H resonances are tuned simultaneously on channel 1. The second channel is reserved for X-band nuclei, so I need to run the entire sequence on channel 1 alone. The standard Varian 19F-1H HOESY sequence (e.g., from BioPack) uses channel 1 for 1H and channel 2 for 19F, which doesn't match my probe configuration.
Has anyone successfully modified or created a 19F-1H HOESY pulse sequence for a single-channel setup on a Varian system? If so, could you share the pulse sequence code or parameter settings (e.g., how to handle frequency switching between 19F and 1H on channel 1)? Any advice on calibrating pulse widths or setting up VNMRJ parameters for this configuration would also be greatly appreciated.
My system is with this probe is a 400 VNMRS. Thanks in advance for any help or pointers to relevant resources!
Best, Shawn

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