Hi John,
When you right click on the spectrum and select and then edit contour levels
etc., those edited parameters will not be saved if you do wpar. To save your
desired contour level parameters, you need to go into ProcPars and scroll
down/choose miscellaneous from the left-side menu and adjust them there
(LEV0, NLEV, and TOPLEV). Then when you do wpar they will be saved.
To apply your desired contour parameters which you have saved with your parameter
set, you have to do levcalc after xfb, which can be part of a macro if you
are doing it from TopSpin or put it into the au processing program (the one listed
in ProcPars under automation) if you prefer icon to process your spectra how you
want. (Btw, I don't think the automatic processing has set contour levels usually
in the default au programs, but you can certainly set them there if you want to.)
I believe this should work now, sorry for not getting it right the first time.
Regards,
Karel
________________________________________
From: Bob Berno <bberno_at_mcmaster.ca>
Sent: 11 November 2023 17:59:48
To: main_at_ammrl.groups.io
Subject: Re: [AMMRL] Contour levels in Topspin
Hello John,
There are three key parameters to define contour levels in TopSpin:
* nlev - number of contour levels (I find 12 - 15 work quite well in most cases)
* lev0 - where to set the lowest contour level, expressed as a percent. For example,
if the signal to noise of your data are good, then set lev0 to something like 20 or 30 percent.
If you have a weak spectrum with rather poor S/N, then set lev0 to something like 8 percent.
If you set it too low, then you end up with too much noise.
* toplev - defines the intensity of the strongest signal. Usually you would set this
to 100 percent (NOTE:when toplev is set to zero, it is assumed you want 100 percent). One
time you might want to set this to less than 100% would be if you have an HSQC spectrum
of a sample with a t-butyl group. Then, you put up with truncating the t-butyl signal
in order to better see the rest of the signals in the spectrum.
Now that you have set those parameters, the next thing you need to do is issue the command
to recalculate the contour levels based upon those parameters.
The command you need to run is: levcalc
After you run levcal, you may need to do a little fine tuning. But you should be in pretty
go shape.
I hope this helps.
Bob.
On 2023-11-10 6:22 p.m., prashansa agrawal via groups.io wrote:
Hi John,
If you right click on the 2D spectra, it give option to edit contour level.
That window will show what is there as default and you can edit it as per
your requirements.
Thanks
Prashansa
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