Hello John,
There are three key parameters to define contour levels in TopSpin:
* nlev - number of contour levels (I find 12 - 15 work quite well in
most cases)
* lev0 - where to set the lowest contour level, expressed as a
percent. For example, if the signal to noise of your data are good,
then set /lev0/ to something like 20 or 30 percent. If you have a
weak spectrum with rather poor S/N, then set /lev0/ to something
like 8 percent. If you set it too low, then you end up with too
much noise.
* toplev - defines the intensity of the strongest signal. Usually you
would set this to 100 percent (NOTE:when /toplev/ is set to zero, it
is assumed you want 100 percent). One time you might want to set
this to less than 100% would be if you have an HSQC spectrum of a
sample with a t-butyl group. Then, you put up with truncating the
t-butyl signal in order to better see the rest of the signals in the
spectrum.
Now that you have set those parameters, the next thing you need to do is
issue the command to recalculate the contour levels based upon those
parameters.
The command you need to run is: *levcalc*
After you run *levcal*, you may need to do a little fine tuning. But
you should be in pretty go shape.
I hope this helps.
Bob.
On 2023-11-10 6:22 p.m., prashansa agrawal via groups.io wrote:
>
>
> Hi John,
> If you right click on the 2D spectra, it give option to edit contour
> level. That window will show what is there as default and you can edit
> it as per your requirements.
>
> Thanks
> Prashansa
>
> On Fri, Nov 10, 2023 at 3:11 PM John H Grimes Jr. <jgrimesjr_at_uga.edu> wrote:
>
> Greetings Spinlanders,
>
> Any time we acquire 2D data, the first thing we always have to do
> is reset the # of contour levels and spacing as the defaults in
> Topspin are too course (level increment 1.8 with 8 levels). I
> have searched for where the global default values are set but have
> been unable to find where this value is stored. Does anyone know
> where to set these values such that they default to whatever
> values we specify?
>
> Thanks for any help,
>
> John
>
> John Grimes
> Complex Carbohydrate Research Center
> University of Georgia
> 315 Riverbend Road, Athens, GA 30602
> (706) 542-4483
> jgrimesjr_at_uga.edu <mailto:jgrimesjr_at_uga.edu>
>
--
Dr. Bob Berno
Manager, NMR Facility
McMaster University, ABB-156
1280 Main St. West
Hamilton, ON L8S 4M1
T: 905-525-9140 x24158 or x24686
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