Dear Colleagues,
We are looking for pulse sequences for 15N and/or 13C relaxation dispersion experiments that will work well with Bruker Avance III consoles and TCI cryoprobes. Do you have such sequences that you are satisfied with for any of the following?
15N relaxation dispersion1 , single quantum 13C methyl dispersion2, multiple-quantum relaxation dispersion3
[1] Tollinger, M., Skrynnikov, N. R., Mulder, F. A., Forman-Kay, J. D., and Kay, L. E. (2001) Slow dynamics in folded and unfolded states of an SH3 domain, J. Am. Chem. Soc. 123, 11341-11352.
[2] Lundstrom, P., Vallurupalli, P., Religa, T. L., Dahlquist, F. W., and Kay, L. E. (2007) A single-quantum methyl 13C-relaxation dispersion experiment with improved sensitivity, J. Biomol. NMR 38, 79-88.
[3] Korzhnev, D. M., Kloiber, K., Kanelis, V., Tugarinov, V., and Kay, L. E. (2004) Probing slow dynamics in high molecular weight proteins by methyl-TROSY NMR spectroscopy: application to a 723-residue enzyme, J. Am. Chem. Soc. 126, 3964-3973.
Thanks in advance for any help you can offer.
Shaokai Jiang and Steve Van Doren
Received on Wed Mar 11 2015 - 06:02:11 MST