Hi Tracy,
Is your probe temperature regulation OK and warm enough- this behaviour could
be due to the sample freezing in the probe, which can be more easily done with
DMSO of course (although that doesn’t explain why the standards seem ok…)?
Best regards
Tim
Tim Claridge
Associate Director, NMR Analytics
Exscientia
> From: main_at_ammrl.groups.io <main_at_ammrl.groups.io> on behalf of Zhang, Tracy Chunxin (GE Aerospace, US) via groups.io
> Date: Friday, 12 January 2024 at 18:07
> To: main_at_ammrl.groups.io <main_at_ammrl.groups.io>
> Subject: [AMMRL] DMSO-D2O sample lock issue
Dear all,
I have run into an odd issue here: one of the chemists using my walk-up instrument,
a 500MHz Bruker Avance III, said she had trouble locking her sample in a DMSO-D2O
mixture (80/20) in ICON. When I tried to run it manually, you can see the lock
signal (we used to call them “rabbit ears” in grad. School) sweeping
in the lockdisp window when you first dropped the sample in the magnet, but a few
seconds later, the lock signal turns into flat noise and the sample would not lock.
A D2O, DMSO, and DMSO/D2O(80/20) standards all worked fine. The sample worked fine
on our Agilent 600MHz NMRS. The chemist could not think of anything in her sample
that might have possibly caused the problem. Just wonder if any of you have seen
something similar and if there is a work around. We would prefer for her to be
able to run her own samples on the walk-up instrument. Thank you!
PS—Wish I could attach the short video of the disappearing lock signal, but
our firewall blocks any external email attachments. ☹
Tracy Zhang, PhD
Analytical Chemist
GE Aerospace
T +1 518 387 7642
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Received on Fri Jan 12 2024 - 10:32:25 MST