Hi all,
We have an oxidation product of ethidium bromide. One of the amino
groups has been oxidized and has experienced an ~+215 ppm shift relative
to the amino 15N shift. The new 15N shift is -100 ppm relative to the
15N shift of the nitro group of nitro-methane. I suspect the new shift
might correspond to the 15N shift of a nitroso group, but I have not
been able to observe the 1H/15N HMBC resonance of a nitroso group in
several standard compounds, including nitroso benzene.
Can anyone tell me if the absence of the 15N resonance of nitroso
benzene is due to intermediate exchange between the monomer and dimer
forms of nitroso benzene?
Is a -100 ppm 15N shift relative to nitro-methane indicative of a
nitroso group?
The spectra were obtained in DMSO.
Thanks,
Eugene
--
Eugene DeRose, Ph.D.
NMR Laboratory Manager
National Institute of Environmental Health Sciences
ITSS Contractor
Phone: 919-541-1981 Fax: 919-541-5707
Received on Fri Jan 05 2007 - 11:45:20 MST