Dear Spinlanders,
I am using a Varian Mercury 400 and Inova 600 for quantitative experiments
and would like to run samples at differing concentrations of a single compound,
measure the integral of one peak in each experiment and determine how well
this approach works for quantitation (no reference internal standard). I also
want to run arrayed experiments and integrate peaks and determine how the
populations are influenced by the experimental parameters. I know that there are
many integration commands available (dpirn, dpir, liamp, dli) but some, maybe
all of these, have referencing variables that could interject problems.
Has anyone done this kind of work and does anyone know which command(s) are
used so that you can integrate a peak(s) and the integral value you get is
truly proportional to the nuclei population? Are there parameters or variables
that must be set (or deactivated) to do this kind of work? I do know that
receiver gain must remain constant from sample to sample and experiment to
experiment.
Thanks for your help.
Patrick Hays
Received on Fri Jan 14 2005 - 13:23:31 MST