Hello all, we're doing our first foray into measuring residual dipolar constants
(RDC's) in organic solvents (chloroform) and are using the commercially available
alignment medium PBLG (Poly-ã-benzyl-Lglutamate
(PBLG, 150,000-350,000 Da, Sigma-Aldrich catalog No.: P5136). We started at 12% w/v.
Had no real problems mixing by using a low-speed centrifuge, going back and
forth 10x.
However the NMR spectra are terrible, and it's hard to even get a decent lock
signal to shim on.
From the literature, I see that some use a capillary filled with a suitable
lock solvent (such as DMSO-d6), but most make no mention of using a capillary.
Any advice would be greatly appreciated.
P.S. Had no problem getting great RDC data in aqueous solution on a small
protein using in C8E5/n-octanol alignment media back in the mid 2000's
Sincerely,
Jim Baleja
--------------------------
Jim Baleja
Pronouns: he, him, his
Department of Medical Education (MV525S)
Department of Developmental, Molecular, and Chemical Biology (lab:Room MV61=
4, rear)
Tufts University School of Medicine
136 Harrison Avenue
Boston, MA 02111
Tel: 617-636-6872
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Received on Wed Feb 28 2024 - 09:45:22 MST