[AMMRL] 13C HMBC of metabolites from a culture with uniformly labelled 13C culture

From: Martha Morton via groups.io <marthadmorton=gmail.com_at_groups.io>
Date: Mon, 01 Dec 2025 12:43:51 -0800

Hi All,

I'm baffled by some signals in an HMBC (not decoupled). The sample is a
metabolomics sample where cells were fed uniformly labeled 13C glucose,
then harvested after an hour. I've seen data like this before, but assumed
I had overlapping signals that were labeled and had 13C-13C, as well as
13C-1H residual couplings. The lactate signal for the CH3 group shows
coupling to the CH2 for endogenous glucose and the labeled glucose. It
also shows extraneous couplings near the C=O in almost a J-resolve
pattern. What am I missing?

Thanks,
Martha


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Received on Mon Dec 01 2025 - 12:46:05 MST