Hi Tom,
If you can run 3d topshim you should be able to get the 1d calibration done
--you do both with the sucrose sample and both use proton to shim instead of
the usual 2H shimming.
The "no signal at all" error is odd--you should have plenty of 1H signal.
I would put the sucrose sample in and open a new dataset and read in the
parameter set gradshim1d1h. Acquire normally (lock, shim, getprosol, etc.
if it will let you shim. If it doesn't shim, the gradient profile might
be ugly but you should still see something) and process the data with fmc so
you see the magnitude spectrum. You should see a reasonable gradient
profile (one Varian guy I know calls it the haystack.) =
If you see a sharp peak then the gradient didn't fire. If you don't see
anything then it's worth troubleshooting why you don't see 1H signal--from 90%
water you should have plenty of signal.
Another simple check--if you disconnect the gradient cable from the probe and
poke pins A and B on the gradient connector you should see about 1.5 ohms, if
I remember correctly, and it shouldn't be shorted to ground (either the center
pin of the connector or the outer shell of said connector). =
That would also give a similar error, although having it happen between 3d
topshim and 1d is kind of odd.
That's what I've got--hopefully a bit of poking around will get you some useful
information.
Cheers,Mike
On Thursday, August 15, 2024 at 08:46:03 AM MDT, Tom Dickinson via groups.io wrote:
Hi All,
I have a question about Bruker's Topshim application. On a 300
MHz AVIII console, I can successfully 3D shim using the sucrose sample
as the manual describes. The display on the lock changes as expected during
the calibrations and it successfully completes the task. Once 3D has completed,
I've attempted to calibrate with the 1D option for ICON automation etc.
Attempting to run with a deuterated doped d2o or the sucrose sample as well,
I get the following error;
RawCheck- no signal at all
I've tried 1D H1 and H2 calibrations and I get the same error. Has anyone
encountered this error and what was required to fix it?
Thank you,
Tom
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Received on Thu Aug 15 2024 - 08:13:00 MST