Peter- Thank you! A great reference. FYI here is the correct citation
for those AMMRLers that are interested.
Robitaille et al. 1991. J Mag Res. vol 92 pp 73-84.
Best all.
Bob
At 02:50 AM 4/2/2004, Peter Lundberg wrote:
>Dear All,
>
>There is also an excellent article by Robitaille et al in MRM, early 1990s
>I believe. The details just escaped me. The paper includes the pKa values
>and shift limits, which are very important for glycolytic intermediates.
>
>73, Peter
>
>>You can try:
>>1) Multinuclear NMR. Edited by Joan Mason. Plenum Press. 1987
>>2) Physiological NMR Spectroscopy: From Isolated Cells to Man. Annals of
>>the New York Academy of Sciences, Vol. 508, 1987.
>>
>>Hope this helps,
>>
>>Edward
>>
>>On Wed, 31 Mar 2004, Robert Wiseman wrote:
>>
>>>
>>> AMMRLers-
>>>
>>> We have been working with a small cell bioreactor doing phosphorus NMR to
>>> detect chemical potential changes arising from glucose challenges. We
>>> have done some extracts and found many resonances in the sugar phosphate
>>> regions that do not correspond with what we expected. These are likely
>>> glycolytic intermediates I suspect. I have been looking for a good
>>> reference (book or manuscript) that describes the chemical shift positions
>>> of biologically active phosphorus compounds at pH ranges from 6.9-7.1. I
>>> recall a book in the mid to late 80's that had a chapter dedicated to this
>>> that included every biologically active phosphate compound, its chemical
>>> shift and the pKa if it was an ionizable compound. I do not recall the
>>> reference and have been unable to locate the book, does anyone recall this
>>> chapter?
>>>
>>> Any recommendation for a decent paper would also be quite helpful. I
>>> have
>>> found references for phosphorus compounds in general, even some web
>>> sources, but specific glycolytic intermediates were not listed.
>>> Thanks
>>>
>>>
>>>
>>> Robert W. Wiseman, Ph.D.
>>> Associate Professor of Physiology and Radiology
>>>
>>> Shipping and Mailing Address:
>>> Michigan State University
>>> Department of Physiology
>>> 2201 Biomedical and Physical Sciences Building
>>> East Lansing, MI 48824
>>>
>>> email: rwiseman_at_msu.edu
>>> alternative email (for files larger than 500 KByte): rww_at_rad.msu.edu
>>>
>>> office 3198 BPS Building
>>> 517-355-6475 (x-1151)
>>> laboratory 3110 BPS Building
>>> 517-355-6475 (x-1342)
>>>
>>
>>--
>>
>>Edward T. Chainani
>>Chemistry Department &
>>National Chemistry Instrumentation Center (NCIC)
>>Ateneo de Manila University
>>Loyola Heights, Quezon City
>>Philippines
>>
>>phone: (632) 426-6001 loc. 5628
>>FAX: (632) 426-1323
>>e-mail: etch_at_chem.admu.edu.ph
>
>
>--
> _at_ _at_
> ..
>==================================================ooOO==========OOoo======
>| Peter Lundberg Ph: (+46 13) 22 27 90 |
>| MR-Unit Fax: (+46 13) 22 27 92 (alt 22 47 49) |
>| Dept of Radiation Physics Email: Peter.Lundberg_at_imv.liu.se |
>| University of Linkoping |
>| S-581 85 Linkoping Efax: PeterLundberg_at_f013222792.fax.sunet.se |
>| Sweden www: http://www.imv.liu.se/radiofysik/ |
>==========================================================================
>() "To a poet nothing is useless." (SJ) ooOO OOoo
>
Robert W. Wiseman, Ph.D.
Associate Professor of Physiology and Radiology
Shipping and Mailing Address:
Michigan State University
Department of Physiology
2201 Biomedical and Physical Sciences Building
East Lansing, MI 48824
email: rwiseman_at_msu.edu
alternative email (for files larger than 500 KByte): rww_at_rad.msu.edu
office 3198 BPS Building
517-355-6475 (x-1151)
laboratory 3110 BPS Building
517-355-6475 (x-1342)
Received on Sun Apr 04 2004 - 21:47:25 MST