Dear All,
There is also an excellent article by Robitaille et al in MRM, early
1990s I believe. The details just escaped me. The paper includes the
pKa values and shift limits, which are very important for glycolytic
intermediates.
73, Peter
>You can try:
>1) Multinuclear NMR. Edited by Joan Mason. Plenum Press. 1987
>2) Physiological NMR Spectroscopy: From Isolated Cells to Man. Annals of
>the New York Academy of Sciences, Vol. 508, 1987.
>
>Hope this helps,
>
>Edward
>
>On Wed, 31 Mar 2004, Robert Wiseman wrote:
>
>>
>> AMMRLers-
>>
>> We have been working with a small cell bioreactor doing phosphorus NMR to
>> detect chemical potential changes arising from glucose challenges. We
>> have done some extracts and found many resonances in the sugar phosphate
>> regions that do not correspond with what we expected. These are likely
>> glycolytic intermediates I suspect. I have been looking for a good
>> reference (book or manuscript) that describes the chemical shift positions
>> of biologically active phosphorus compounds at pH ranges from 6.9-7.1. I
>> recall a book in the mid to late 80's that had a chapter dedicated to this
>> that included every biologically active phosphate compound, its chemical
>> shift and the pKa if it was an ionizable compound. I do not recall the
>> reference and have been unable to locate the book, does anyone recall this
>> chapter?
>>
>> Any recommendation for a decent paper would also be quite helpful. I have
>> found references for phosphorus compounds in general, even some web
>> sources, but specific glycolytic intermediates were not listed.
>> Thanks
>>
>>
>>
>> Robert W. Wiseman, Ph.D.
>> Associate Professor of Physiology and Radiology
>>
>> Shipping and Mailing Address:
>> Michigan State University
>> Department of Physiology
>> 2201 Biomedical and Physical Sciences Building
>> East Lansing, MI 48824
>>
>> email: rwiseman_at_msu.edu
>> alternative email (for files larger than 500 KByte): rww_at_rad.msu.edu
>>
>> office 3198 BPS Building
>> 517-355-6475 (x-1151)
>> laboratory 3110 BPS Building
>> 517-355-6475 (x-1342)
>>
>>
>
>--
>
>Edward T. Chainani
>Chemistry Department &
>National Chemistry Instrumentation Center (NCIC)
>Ateneo de Manila University
>Loyola Heights, Quezon City
>Philippines
>
>phone: (632) 426-6001 loc. 5628
>FAX: (632) 426-1323
>e-mail: etch_at_chem.admu.edu.ph
--
_at_ _at_
..
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Received on Fri Apr 02 2004 - 14:54:08 MST