Hello everybody,
we trying to run a 3D HNCO according to Kay, Xu and Yamazaki, J.Mag. Res.
109, 129-133 (1994) and for some reason in a 3D transform some (20-30% or
so) of the crosspeaks when looked at in 15N-13C planes look cosy-like
(antiphase with 4 components). All cross-peaks look absorptive in 1H-13C
and 1H-15N planes, and in 2D transforms. The protein is ~18kDa, we run on
500 Avance at 298K.
Has anybody else encountered this or has a fix for it? Thanks a lot for
your help.
Ryszard Michalczyk, Ph. D.
Bioscience Division
Leo Szilard Resource
B-3; MS G758
Los Alamos National Laboratory
Los Alamos, NM 87545
phone (505) 667-7918
fax (505) 667-0110
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The most universal quality is diversity -- Montaigne
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Received on Mon Sep 15 2003 - 15:01:45 MST