Hi all,
I'm supporting a protein research group that needs to work at low concentrations
(20-50 uM) and is asking about using a 10mm cryoprobe. I've talked about line
shape and shimming on such probes, and also mentioned 8mm probes. I'm uncertain
about how bad Q-spoiling by buffers might be. I've used X-direct-detect 10mm
probes, but have only talked with vendors about 8mm probes and those discussions
were at least 10 yrs ago. What I recall is that 5mm probes are better optimized
for both line shape and sensitivity, and that overcomes (at least most of) the
advantages of larger sampling volume with the bigger diameter probes. But I've
always wondered how much the economics of such expensive devices -- a larger
diameter probe having considerably narrower utility -- might influence such
discussions. One aspect is whether new shimming technologies might make the larger
diameter probes more competitive than they used to be?
The research group remains interested in the possibilities. I agreed to ask
in this forum who might have such probes, especially those that would be open
to outside academic use. Even if you have a probe that is not available for
use to others, it would be great to hear more about their utility for 1H- and
13C-detected 2D and 3D experiments.
Cheers,
Charlie
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Received on Mon Oct 28 2024 - 11:46:11 MST