Date: Tue, 15 Oct 2024 18:37:02 +0000
Hi Ken,
To follow up on Bob's great comments and suggestions, we had an experience
about 6 months ago with our B500 Prodigy involving sudden huge ADC overflow
problems on CH-HSQCs (but not the problem you describe with cool down after
fills....for us, it consistently takes 20min to get cold after a dewar fill
+ 10 min to reach a stable N2 pressure). Our ADC issues occurred during
13C expts. 1H and 15N were fine.
In our case, it turned out there were a few slightly worn O-rings in the line +
cryoplatform compromising the vacuum enough to lead to micro arcing in the CPP.
The change in vacuum is difficult to discern in the vacCRP metric.....that
only changed a couple of mV.
Bruker ultimately installed a Penning gauge on the outside of the Prodigy
cryoplatform. It should read in the —7-8e^-7 range for the vacuum when
the system is operating correctly. You can track it in the cryo service
GUI after it is operating (it appears as S12).
Good luck!
J
----------------------------------------------------
James Aramini, Ph.D.
Senior Research Associate
Dept. of Molecular Genetics
Rm 1233, Medical Sciences Building
University of Toronto
1 King's College Circle
Toronto, ON M5S 1A8
Cell: 437-326-6427
Phone: 416-978-6229
----------------------------------------------------
> From: main_at_ammrl.groups.io on behalf of Bob Berno via groups.io
> Sent: Tuesday, October 15, 2024 12:48 PM
> To: main_at_ammrl.groups.io
> Subject: Re: [AMMRL] AMMRL: Prodigy Error E2321 Rapid Buffer Vacuum Decay. Puming Non Stop
Hello Ken,
It should not take two hours after a regular fill for the system to be
operational again. There is definitely some kind of problem.
I have never seen that error pop-up message. It does suggest that there
is a leak, but it could be a red herring.
I have a few questions:
Do you see any condensation or other signs of cold spots on the nitrogen
transfer line going from the Dewar to the Prodigy?
Have you seen an increased rate of liquid nitrogen consumption?
Can you bring up the "logs" from the past several weeks? Have a look at
the operating temperature - but check other parameters as well, especially
last night when the long carbon experiments were running?
The fact that they were long carbon experiments shouldn't be a problem.
I'd be more concerned about long HSQC experiments with 13C-decoupling.
Good luck.
Bob.
On 2024-10-15 11:36 a.m., Kenneth Sharp-Knott via groups.io wrote:
Has anyone ran across this error on their Prodigy instruments?
I've had several people say they ran long carbons last night and had little
to no signal. There did appear to be more Receiver Overflow errors than usual.
The probe is still cold and a quick 16 scan carbon this morning shows strong
signal.
E2321: Rapid Buffer Vacuum Decay. Pumping Non-Stop.
On a related note: We have had issues cooling down after refills for the
last several months. Tightening the screws at the Fill and Exhaust ports on
the Prodigy Dewar neck seemed to help, but the last time took almost two
hours to go back to cool. Bruker suggests a leak, but I'm not sure where
it is.
Ken Sharp-Knott
Manager of Analytical Services and the NMR Facility
Department of Chemistry
Virginia Tech
(540)267-6502 (Cell)
(540)231-0885 (Office)
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