Hi,
I've been trying to get saturation difference (and waterlogsy) experiments
running on our Bruker Avance 600. We have topsin 2.1 (May 14, 2013)
patchlevel 8.
A variety of STD pulse sequences are included in the
/opt/topspin/exp/stan/nmr/lists/pp directory
ie stddiff stddiffesgp stddiffgp19 ( .2 .3 for each) These are all dated
Nov 2014... when the system was put back into use after some work on the
magnet.
However, there are no corresponding parameter files in
/opt/topspin/exp/stan/nmr/par
Some were added into the /opt/topspin/exp/stan/nmr/par/user
directory later, however I cannot see that anyone ever actually used them
(no data saved anywhere using any of the pulse sequences), and attempting
to load and use any of these parameter sets does not give me any kind of
signal- and in fact the starting values are all kind of useless, as far as
I can figure. Nor does it automatically route things correctly (you have
to do edasp to get rid of a bunch of errors)- maybe that is normal?
I'm a longtime varian user, so trying to wrap my head around how
everything works (ie in vnmrj I have the pulse sequence and parameter set
included in the software, so I calibrate my proton 90, select the
experiment I want from the experiment menu, and after choosing my
saturation frequencies and time away I go with no trouble at all).
I've looked at the shaped pulses, and I think I've got sorted on how to
pick a power level based on my high power proton 90- the values all seem
reasonable to me.
My other problem is that when I have tried opening sample data sets that
people have given me, the software complains about various things like
routing, digitizer types etc, and they only way I can see what pulse
lengths delays etc they used is to open up the big tables with all the p
values etc. which is kind of painful to work through.
Any pointers, parameter sets that work on exactly the same configuration
would be greatly appreciated!
Thanks,
Tara
*********************************************
Dr. Tara Sprules
Quebec/Eastern Canada High Field NMR Facility
www.nmrlab.mcgill.ca
phone: (514) 398-1721
fax: (514) 398-8254
3420 University St., Rm 023
McGill University
Montreal, QC, H3A 2A7
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Received on Fri Oct 16 2015 - 05:39:09 MST