Dear Bruker users,
We have a 600 MHz equipped with cryoprobe. I observed
something really puzzled me. Basically, the offset
shaped pulse on 13C commonly used for triple resonance
experiments reduce the sensitivity to a different
extent dependent randomly on the offset values. I
don't know exactly what causes this, and would like to
know anyone observed similar thing on Bruker machine
or not, and how to resolve this problem?
Here are what I did observe:
I used a 13C-labeld methanol in DMSO to run a series
of 13C-HSQC with hard pulse and soft pulses.
With on resonace shaped pulse, the intensity from both
hard and shapled pulses are the same. As long as
offset pulses are used, the intensity dropped to a
different extents with no correlation with the values
of offset. The shaped pulses are commonly used like
Q3/Q5 etc.
I requested to have a new SGU board, but I am not sure
will that fix the problem or not.
Anyone out there has ideas what is going on?
Best Regards!
Dr. Weidong Hu
Immunology Division, BRI, City of Hope
1500 Duarte Rd., Duarte, CA 91010
Tel: 626 359-8111, ext: 63416
Fax: 626 301-8186
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Received on Mon Oct 31 2005 - 19:30:23 MST