1. use large gain 63dB
2. Set pulse width to 20us start with (about 30 degree pulse)
3. Turn the decoupler on for NOE enhancement
4. use big sw, than move f1, make sure signal is not folt in.
5. check the pulse delay. lb=1hz, T1 =0.5s to 170s
If you have more questions, call me.
Shaoxiong Wu
Emory University
404-727-6621
shiulong@bayou.uh.edu writes:
>We have a GE QE-300 with a BB probe here and I am trying to develop an
>experimental method to do natural abundance N-15. I have not work with
>N-15 before so I don't have a feel for it. I am using an 80% DMF in
>acetone-d6 standard and an N-15 enriched NH4Cl (1.6 mg) standard with no
>success. Does anyone have an SF, SW and NA I could start with? It would
>also be helpful to know which wand I should use. Can our probe actually
>tune and match to that frequency (30.5 MHz)? The probe seems to tune and
>match with the SH wand. Does SH mean short? None of our other wands seem
>to work. Any help with this problem will be accepted if I can't do this
>on
>the QE I will have to move the project to the AMX-600 which is already
>tight on time.
>
>
>Thank you
>
>chuck
>
>
>Charles L. Anderson, Ph.D.
>Manager NMR Facilities
>Department of Chemistry
>University of Houston
>Houston, TX 77204-5641
>Office: (713)-743-2728
>Fax: (713)-743-2709