Pat,
Yes and no (but mostly no). Unlike chromatographic peaks (GC), the line
widths of NMR peaks are not identical, hence, total area under the peak is
dependent upon the peak height and the line width, which is a function of
relaxation behavior of the nucleus under investigation). You may look into
Chapter 9 on Practical aspects of intensity measurement and quantitative
analysis in "Practical NMR Spectroscopy by Martin, Delpuech and Martin
(Heyden) 1980.
Sadanand
Sadanand V. Pathre, Ph. D.
Senior Research Specialist
3M Pharmaceuticals
270-4S-02
Tel: 651-733-6453
Fax: 651-7363974
email: pathresv_at_mmm.com
<Natalie.Serkova_at_
UCHSC.edu>
To
03/27/2006 11:11 <pahays_at_earthlink.net>
AM <ammrl_at_ammrl.org>
cc
Subject
RE: Peak Height NMR Quantitation
Pat:
Do you mean doing a peak peaking function instead of integration?
If so, some people did it for 13C-NMR (only - as far as I know).
Natalie
Dr. Natalie Serkova
Assistant Professor of Anesthesiology and Radiology
Biomedical MRI/MRS Cancer Center Core
University of Colorado Health Sciences Center
phone: 303 315 1878
pager: 303 266 2910
-----Original Message-----
From: Pat Hays [mailto:pahays_at_earthlink.net]
Sent: Monday, March 27, 2006 3:11 AM
To: AMMRL
Subject: Peak Height NMR Quantitation
Dear AMMRL:
Does anyone know of any articles that discuss using peak height of
proton NMR spectra for quantitation?
Thanks,
Pat Hays
Received on Tue Mar 28 2006 - 18:00:23 MST