Hi Geoff,
That was a super helpful suggestion, thank you. I pulled the entire histlog
and fed it into an AI assistant, gave it the timestamps of the failed
experiments, and it nailed the exact sequence of events.
For anyone who might run into this in the future, here is what happened under
the hood:
It turns out a TopSpin restart triggered a backend communication issue where
the system failed to check out its CodeMeter license (CmContainer Entry not
found). This caused a cascade of permission issues—specifically, Edprosol
started throwing repeated I/O errors (IoError:1.0) because it couldn't delete
remote prosol/pulseassign directories.
Because of this backend corruption, the system was completely unable to generate
Non-Uniform Sampling (NUS) lists. When the experiments reached the receiver
gain adjustment (rga) step, the NUS sampler failed (callNusSamplerError: Generation
of NUS-list failed!). Without the NUS list, the pulse program compiler couldn't
resolve the SPARSELIST variable, resulting in syntax errors that aborted the run.
This perfectly explains why our 1D experiments were running fine, but every
single 2D experiment was failing—we run all of our 2Ds with NUS.
Thanks again for pointing me in the right direction! Looks like a reboot
is in order... Not sure what triggered the issue to begin with.
Ken Sharp-Knott
Manager of Analytical Services and the NMR Facility
Department of Chemistry
Virginia Tech
(540)267-6502 (Cell)
(540)231-0885 (Office)
________________________________
> From: main_at_ammrl.groups.io on behalf of Akien, Geoffrey via groups.io
> Sent: Friday, July 10, 2026 10:15 AM
> To: main_at_ammrl.groups.io
> Subject: Re: [AMMRL] 2D Experiments all failing on Bruker Neo500 w/Prodigy
Hi Ken,
In the event you’re still running the same session of TopSpin you can
sometimes find stuff in the hist logs, but I tend to find it quite hard work.
As for the helpful “Acquisition Failed” in IconNMR – this appears to =
be a bug in 5.0.0 where even if you have the “Show all TopSpin Warnings”
\option ticked, it won’t list errors occurring during the acquisition
AU program run from AUNM. Very annoying to deal with. Hopefully this gets
fixed at some point?
Thanks
Geoff
> From: main_at_ammrl.groups.io On Behalf Of Kenneth Sharp-Knott via groups.io
> Sent: 10 July 2026 14:48
> To: main_at_ammrl.groups.io
> Subject: [External] [AMMRL] 2D Experiments all failing on Bruker Neo500 w/Prodigy
Overnight, about a dozen 2D experiments (HSQC, COSY, HMBC) all failed with =
no error other than Acquisition Failed. In each case a 1H and in most cases
a 13C experiment was run successfully before the 2D's. Running Topspin
5.0.0 without (major) issues since release on all of our instruments.
Is there any log that might shed light into why the experiment's failed?
Any ideas?
Thanks in advance.
Ken Sharp-Knott
Manager of Analytical Services and the NMR Facility
Department of Chemistry
Virginia Tech
(540)267-6502 (Cell)
(540)231-0885 (Office)
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Received on Fri Jul 10 2026 - 08:08:10 MST