Dear Bruker Users,
I would like to run the zghc on Bruker AV console with topspin 2.1 so that I can decouple the Ha region from the Hn for the peptide sample.
Somhow, I could only get a few spikes for the spectrum. Could anyone share your successful experience in using zghc sequence?
Thank you in advance.
Here are some related parameters I used for the testing, I am not sure what other parameters or settings I should pay attention to.
1H channel 1 and 2 are routed to SGU2, then to proton amplifier
HDDUTY is 20%
HDRATE 20
DW 83.2us
CPDPRG2 set to mlevsp180.p31
Shaped 180 is Q3 with 64 points, and duration is 4500us
Pl24 power for shaped 180 Q3 is 35.6dB
O2p at 4.45ppm
O1p at 4.05ppm
DECIM 32
DIGMOD digital
The hd from bruker cpd library is
1 d31
P31:0
Jump to 1
Regards,
Weidong
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Received on Tue Feb 05 2013 - 09:53:47 MST