RE: S/N of CryoProbe

From: Mike Delk <mid15+_at_pitt.edu>
Date: Tue, 31 Jan 2006 13:36:44 -0500

Dear Youlin,

 

I must apologize for giving you bad information. In my replying to quickly
I have given you the wrong numbers. Below are the correct ones so please
correct you chart.

 

800 Cryoprobe ethyl benzene sino - 8680:1 and the Sucrose is 884:1

 

800 standard QXI probe is 1809:1 and 380:1

 

Mike

 

====================================================

Michael J. Delk

NMR Lab Manager

Structural Biology Program

University of Pittsburgh

B033 Biomedical Science Tower 3

3501 Fifth Avenue

Pittsburgh, PA 15260

412.383.9802 Phone

412.648.9008 Dept. Fax

mid15_at_pitt.edu

 

-----Original Message-----
From: Youlin Xia [mailto:yxia_at_uh.edu]
Sent: Monday, January 30, 2006 7:18 PM
To: ammrl_at_ammrl.org
Cc: yxia_at_uh.edu; xxie_at_uh.edu; glegge_at_uh.edu
Subject: S/N of CryoProbe

 

Hello, dear NMR Managers,

 

In last Saturday, I emailed to AMMRL to know the S/N of CryoProbe. I
obtained quite a few replies. The results are listed in the following table.

 


Institution

Instrument

S/N


University of Connecticut

Varian 600

4000:1


3M Pharmaceuticals

Bruker 700 TXI

7400:1


University of Pittsburgh

Bruker 800

6036:1


University of Chicago

Varian 600

5100:1


UT-Houston

Bruker 600

4500:1


University of California, Irvine

Bruker 500 TCI

5453:1


University of Houston

Bruker 800 TCI

6400:1

 

 

Additionally, a few friends suggested me pay attention to decoupling
experiment, temperature limits, experiments with proteins, et al. Thank you
all so much.

 

Today the engineer got another number, the S/N of the peak around 5.4 ppm of
Sucrose (2 mM Sucrose with 0.5 mM DSS, 2 mM NaN3 in 10% D2O and 90% H2O),
which is 495:1.

 

I'm very depressed with this number because the S/N of the same sample with
our room temperature probe TXI is 467:1 although the engineer said he can
boost the S/N to 800:1. Even if the S/N is 800:1, the enhancement is only
1.7-fold. So much money spent is only for the 1.7-fold S/N enhancement?

 

Please tell me what the S/N is with Sucrose sample in your cryoprobe.

 

Thanks in advance,

Youlin Xia

Manager NMR Facility

University of Houston

Houston, TX
Received on Wed Feb 01 2006 - 16:14:02 MST

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