Hello All
I have a situation in my Bruker DMX750 spectrometer where, in a
repetition of three attempts, the user lost lock when a overnight run
of NOESY or TOCSY was setup and this was on a sample that contains 99.9
% D2O. When we come back in the morning, sure enough the lock is lost
and whe we look at the interferogram, using the residual water at 4.7
ppm as a focal point, the line just drifts monotonically to one side,
similar to what we will observe by recording a water peak without
locking the system over a long period of time.
Normally on this instrument, mostly biological samples with 10% D2O
buffers are employed and that is why I am getting the doubt as to if
'too much lock signal ' can cause the lock system unstable and make it
loose the lock.
Thank you as always for sharing your experiences and insights.
Sincerely
Rajan
--
*_______________________________
Rajan K Paranji, Ph.D.
*NMR Facility Manager
Department of Chemistry
Room 65, Bagley Hall
Univeristy of Washington
*Seattle, WA 98195
ph: 206 685 2581
fax: 206 685 8665
email: paranji_at_chem.washington.edu
___________________________________*
Received on Wed Aug 17 2005 - 19:43:56 MST