Dear Colleagues,
thank you very much for all your help and response regarding our question
on how to run 2D experiments using MSL console and DISMSL software. We got
several messages from different people (Dr. John Decatur, Dr. Hanno Leisen,
Dr. Tanja Pietraß, Dr. Bob Taylor, Dr. Sambasivam Sendhil Velan), and all
the information has been extremely useful. Thank you very much for taking
your time to write and talk to us.
To summarize the responses:
1. It is definitely possible to run 2D experiments using MSL 200 console
and DISMSL software.
The original software contains a library of standard 2D pulse sequences and
one can borrow the synthax from some of the programs.
The experimental setup is performed by introducing a variable loop counter
during the evolution period which is incremented in a similar fashion to
the newer Bruker systems that utilize XWinNMR software. Another way of
incrementing the evolution period is by using a VD command within the
pulse program and then giving a delay list at the end. One can type in the
list of the delays depending on the F1 spectral width.
An easy quick way to get used to setting up 2D experiments is to run a
quick COSY. Set up a 1D 1H spectrum. Use the pulse program COSYN.PC and
follow the comments in the pulse program. The data is acquired with the AU
progrm TWODAQ.AUM with the data stored as a serial file, filename.SER.
Before processing give a ST2D command to bring up the 2D parameters. Adjust
as desired. An XFB will Fourier transform in both dimensions. Then either
EP2D (or AP2D depending upon whether or not you have an array processor in
your Aspect) will allow you to expand the processed data.
Please note that some pulse programs use buffered acquisition and will NOT
use an AU program for acquisition. The data is then stored after
acquisition with the WALL filename.SER command (Write ALL).
2. There seem to be a general consensus regarding the data processing: the
best way is to convert the Aspect3000 data into the PC-compatible format
which can be then transferred into a PC and processed using any of the
available data processing software. There are several options on how to
convert the data and transfer them to the externally connected PC.
The quick and expensive way is to purchase an OpenAspect Data Transfer
Package from MR resources.
There is a longer but extremely cost effective approach (it will cost a
total of ca. $100 plus the cost of an old PC). This was a really helpful
advice from Dr. Tanja Pietraß who in turn learned about this from Dr. Dave
Van der Velde. The recipe is the following:
a. One needs to purchase a computer board and a cable from Computer Boards,
125 High Street, Mansfield, MA 02048. The board has the number: CIO-D1024
and the cable: C37FFS-5 (5 feet long)
The board is ca. $62, the cable ca. $30.
b. The hardware connection for board installation is described in the
article in JMR 88 (1990) 511-522
c. One needs to download a nmrnet software on both the PC and on the
Aspect. If anyone needs the software- please, let me know. Dr. Tanja
Pietraß has nicely sent us the package for the PC, and the diskette for
installation on the Aspect, and I would be happy in turn to send it to
anyone else who is interested.
d. An important detail: the nmrnet software requires an I/O address of 300.
This is usually used as the default for the ethernet address. So change
the one for the ethernet address to 310 or anything else and set the one
for nmrnet to 300.
best regards,
Tatyana Polenova
Received on Fri Aug 25 2000 - 14:19:33 MST